One hundred and twenty eight, 28-weeks-old Lohmann LSL hybrid layers were used in his experiment, which lasted 12 weeks to investigate the effect of propolis supplementation on the productive performance, egg quality traits and haematological variables of laying hens. All hens were randomly classiﬁed into four equal experimental groups, eight replicates (4 birds/each). Hens in group 1 were fed on a commercial diet and considered as control group, while those in groups 2, 3 and 4 were fed on the same commercial diet and supplemented with 250, 500 and 1000 mg propolis/kg diet. The obtained results revealed that daily feed con-sumption/hen increased insigniﬁcantly with increasing propolis level than that of the control group. Regarding the means of egg mass and egg production rate, it was observed that the laying hens fed diets containing 250 and 1000 mg propolis/kg signiﬁcantly (p < 0.05) produced more and heaver egg in comparison with control group. External egg quality traits have not affected with increasing the level of propolis, while eggshell weight was signiﬁcantly (p < 0.05) increased. The internal egg quality traits except albumen and yolk percentages
increased signiﬁcantly (p < 0.05) with increasing propolis level for treated hens as compared to those in the control. Concerning the haematological parameters, the results showed that the levels of total protein and glo-bulin increased signiﬁcantly with increasing propolis level, while cholesterol and liver enzymes were signiﬁ-cantly decreased (p < 0.05). Heterophils count of hens in the treated groups signiﬁcantly decreased, whereas the lymphocyte count signiﬁcantly increased, resulting in a decreased H/L ratio than that of the control group. Thus, it could be concluded that the supplementation of 250 mg propolis/kg diet is highly recommended to improving egg production, blood constituent and haematological parameters of the commercial laying hens.