Mycoflora of Edible- offal of Slaughtered Animals in Qena Abattoirs with The Aid of RAPD- PCR Technique.

Abstract

Eddible- offal of slaughtered animals is considered as popular diete in Egypt, so a total of 50 samples of them were collected from large animals (cattle and buffaloes slaughtered in Qena abattoirs (10 each of heart , intestine, liver, rumen and spleen). Samples were subjected to mycological investigation for isolation and identification of various mould species with the aid of RAPD- PCR (Random Amplified Polymorphic DNA- Polymerase Chain Reaction). Rumen and intestine were heavy contaminated with moulds than other examined edible -offals of the slaughtered animals; 5.3 X 10⁴  ± 0.3 X 10⁴ and 4.1 X 10³ / 1 g± 0.2 X 10³  of the samples, respectively. Fungal contamination was not detected in all samples of heart and spleen. Ninty-eight mould strains were detected from examined samples , the majority of isolates belonging to Aspergillus spp. 23( 23.5 % ), Trichoderma 13( 13.3 % ), Fusarium spp. 12(12.3%) and Absidia corymifera 11(11.2%). The isolated species of Aspergillus species were further confirmed by using RAPD- PCR technique. Harmful effect on consumer health resulting from mould contamination of edible- offal and the hygienic measures adopting in abattoirs were fully discussed