Dr. Ashraf Oukasha Abd El-latif

Assistant Professor - Associate Professor (Insect Physiology), Department of Plant Protection

Faculty of agriculture

Address: Faculty of Agriculture, Sohag Universiy, Sohag, Egypt


2017 | Keywords pyrethroid resistance, Termite,
Pyrethroid Resistance and Its Mechanism in Field Populations of the Sand Termite, Psammotermes hypostoma Desneux.
Termites are eusocial insects that are found on all continents except Antarctica. Termites have serious destructive impact, damaging local huts and crops of poor subsistence. The annual cost of termite damage and its control is determined in the billions globally. In Egypt, most of these damages are due to the subterranean termite species especially the sand termite, P. hypostoma. Pyrethroids ... Read more

Evaluation of some herbicides efficiency on sugarcane crop
The present study was carried out at Kom-Ombo Agricultural Research Station Farm, Aswan Governorate, Agricultural Research Centre, Giza, Egypt, to evaluate the herbicide treatments on different weed species in sugarcane fields and their control methods on yield and quality of sugarcane. The obtained results revealed that, the weed species observed in the sugarcane field were twelve weed species belonging to ... Read more

ITS sequence as barcode for identifying closely related species of Chelonus blackburni Cameron, and Apanteles angaleti Mueseback, a biocontrol agent of cotton ecosystem
The most important point for the success or failure of biological control is the correct identification of the pest or the natural enemy especially when either the pest or the natural enemy has closely related species that are often morphologically indistinguishable differing only in their behavior and physiology. In the current, the ITS sequences (Internal Transcribed Spacer) of two parasitoid ... Read more

Estimation of the annual field generations of Parlatoria blanchardii and prediction of its expected peaks using thermal units accumulation under Luxor governorate condition, Egypt
The date palm scale, Parlatoria blanchardii (Targioni-Tozzetti) is a target pest of date palm trees causing severe damage resulting in early leaves drop and yield reduction. In the current study, the approximated numbers, occurrence date, size of the generations and their peaks of P. blanchardii were determined during two succesive seasons (2010/2011 & 2011/2012) in order to predict the degree ... Read more

Bio-potency of serine proteinase inhibitors from Cowpea (Vigna unguiculata) seeds on digestive proteinases and the development of Spodoptera littoralis (Boisduval)
Serine protease inhibitors (PIs) have been described in many plant species and are universal throughout the plant kingdom, where trypsin inhibitors is the most common type. In the present study, trypsin and chymotrypsin inhibitory activity was detected in the seed flour extracts of 13 selected cultivars/accessions of cowpea. Two cowpea cultivars, Cream7 and Buff, were found to have higher trypsin ... Read more

Isolation and purification of a papain inhibitor from Egyptian genotypes of barley seeds and its in vitro and in vivo effects on the cowpea bruchid, Callosobruchus maculatus (F.).
The cysteine inhibitors that are known as cystatin have been identified and characterized from several plant species. In the current study, 44 barley (Hordeum vulgare) genotypes including 3 varieties and 41 promising lines were screened for their potential as protease inhibitors. The barley genotypes showed low inhibitory activity against trypsin and chymotrypsin enzymes with a mean of 4.15 TIU/mg protein ... Read more

Portease purification and characterization of a serine inhibitor from Egyptian varieties of soybean seeds and its efficacy against Spodoptera littoralis.
Serine inhibitors have been described in many plant species and are universal throughout the plant kingdom. Trypsin inhibitors are the most common type. In the present study, trypsin and chymotrypsin inhibitory activity was detected in the seed flour extracts of four Egyptian varieties of soybean (Glycine max). The soybean variety, Giza 22, was found to have higher trypsin and chymotrypsin ... Read more

In vivo and in vitro inhibition of Spodoptera littoralis gut-serine protease by protease inhibitors isolated from maize and sorghum seeds
Seeds of cereals (Gramineae) are a rich source of serine proteinase inhibitors of most of the several inhibitor families. In the present study, trypsin and chymotrypsin inhibitory activities was detected in the seed flour extracts of three varieties of maize (Zea maize) and six varieties of sorghum (Sorghum bicolor). The maize variety, Hi Teck 2031 and the sorghum variety, Giza ... Read more

Biochemical characterization of the midgut serine proteases of the Egyptian cottonworm, Spodoptera littoralis (Boisduval) and their interactions with standard protease inhibitors
Serine proteolytic activities in soluble protein extracted from the larval midgut of Spodoptera littoralis were studied using specific substrates and protease inhibitors. There are significant differences in serine activities in respect to larval development. The serine activities increase with instar development, reach each maximum at third instar larvae while the lowest serine activities are observed in the fifth instar larvae. ... Read more

Overexpression of cytochrome P450 CYP6B7 mediated pyrethroid resistance in Indian strains of the cotton bollworm, Helicoverpa armigera (Hübner)
The role of cytochrome P450 monooxygenase in pyrethroid resistance was studied in different strains of the cotton bollworm, Helicoverpa armigera, from India. Filed collected strains of Nagpur and Delhi were compared to the laboratory reared population. The results showed a high resistance to deltamethrin, α-cypermethrin, and β-cyfluthrin. The results also showed that this resistance could be reduced by using piperonyl ... Read more