Electronic Exam System is improves examination security, testing practice and reduces examination costs. This study was aimed to assess opinion of teachers' and students' toward implementation of e-exam in Faculty of Nursing at Sohag University. The study subject consists of (11) teachers and (140) student. Data collected through personal interview by using the teachers' opinion questionnaire and students' opinion questionnaire, the whole duration of data collection took about fifteen days after applied the exam. Results: The most of both teachers and students had agreed that, e- exam saves time, saves material costs of printing and other papers and provides self assess for student. And near half of them disagreed that, don't speak of dealing with computer, prefer paper exam, and paper exam more easily. As a result and in the light of the finding, the researchers recommended that; Lecturers  should  be  trained  and  assisted  to  adapt  to  the  new technologies, students should be assisted to acquire basic skills in computer that will help them during the conduct of e-examinations, and provides adequate computers