Tenacibaculosis is a serious bacterial disease known to affect many species of marine fish such as Rhinecanthus assasi (Picasso Trigger fish), Neoglyphieodon meles (Black damsel fish) and Cheilinuslunu latus (Broomtail wrasse). Tenacibaculum maritimum pathogen was recovered from ulcers, livers and spleens of clinically diseased fishes from coral reef in the marine site off the National Institute of Oceanography and Fisheries (NIOF) at Hurghada, Egypt. The obtained isolates were identified as T. maritmum by the morphological and biochemical characterization. The prevalence ratio of Tenacibaculosis among the clinically diseased Black damsel, Picasso Trigger and Broomtail wrasse fishes were 14.3, 13.3 and 19.4% respectively. The highest prevalence levels of the disease in the investigated clinically diseased Black damsel, Picasso Trigger and Broomtail wrasse fishes reached 20, 16.7 and 28.6% during winter and the lowest was during summer (0%). Also, FMM and Huso-Shotts media were the most effective media for the recovery of T. maritimum from diseased fish followed by MA and trypticas soya agar media